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The effectiveness of platelet cold-storage for long-term preservation
- Date2019-05-23 20:30
- Update2019-11-19 19:10
- DivisionDivision of Strategic Planning for Emerging Infectious Diseases
- Tel043-719-7271
The effectiveness of platelet cold-storage for long-term preservation
Cho Yong Gon
Department of Laboratory Medicine, Medical School of Chonbuk National University
Yang Jin Hyuk, Kim Min-Ju, Kim Jun-nyun
Division of Human Blood Safety Surveillance, Center for Organ Transplant Management, KCDC
The room temperature storage of platelets contributes to platelet storage lesion (PSL) and the risk of bacterial growth. To increase shelf life and improve safety in transfusion, an alternative storage method is needed for platelet concentrates (PCs) and apheresis platelets. In a previous study, refrigeration storage of platelets with N-acetylcysteine (NAC) potentially prevented the development of PSL. This current investigation was designed to assess the efficacy of NAC and ascorbic acid (Vitamin C) as anti-platelet compounds during extended PC storage. The use of NAC and ascorbic acid was found to moderate the development of PSL and enhance in vivo recovery of refrigerated platelets in a similar style to PCs stored at room temperature. The positive effect of NAC and ascorbic acid on refrigerated PCs was suggested to be via regulation of reactive oxygen species, platelet activation, and sialic acid channels. The findings of this study might extend PC storage by up to 10 days.
Keywords: Ascorbic acid, Cold-stored platelets, Long-term preservation, N-acetylcysteine, Platelet concentrates
Cho Yong Gon
Department of Laboratory Medicine, Medical School of Chonbuk National University
Yang Jin Hyuk, Kim Min-Ju, Kim Jun-nyun
Division of Human Blood Safety Surveillance, Center for Organ Transplant Management, KCDC
The room temperature storage of platelets contributes to platelet storage lesion (PSL) and the risk of bacterial growth. To increase shelf life and improve safety in transfusion, an alternative storage method is needed for platelet concentrates (PCs) and apheresis platelets. In a previous study, refrigeration storage of platelets with N-acetylcysteine (NAC) potentially prevented the development of PSL. This current investigation was designed to assess the efficacy of NAC and ascorbic acid (Vitamin C) as anti-platelet compounds during extended PC storage. The use of NAC and ascorbic acid was found to moderate the development of PSL and enhance in vivo recovery of refrigerated platelets in a similar style to PCs stored at room temperature. The positive effect of NAC and ascorbic acid on refrigerated PCs was suggested to be via regulation of reactive oxygen species, platelet activation, and sialic acid channels. The findings of this study might extend PC storage by up to 10 days.
Keywords: Ascorbic acid, Cold-stored platelets, Long-term preservation, N-acetylcysteine, Platelet concentrates
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