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Laboratory-based diagnostic test results for human granulocytic anaplasmosis in 2017
- Date2018-06-28 21:03
- Update2018-06-28 21:03
- DivisionDivision of Strategic Planning for Emerging Infectious Diseases
- Tel043-719-7271
Laboratory-based diagnostic test results for human granulocytic anaplasmosis in 2017
Lee Kyeong Ah, Hwang Seon Do, Kang Byung Hak, Kim Jae Ok
Division of Bacterial Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum. Clinical features of HGA range from mild illness such as fever, headache, myalgia, malaise, thrombocytopenia, and leukopenia to severe disease with gastrointestinal and respiratory distress, myocarditis, neurological complications, septic shock-like disease, and even death.
We performed serological HGA tests using immuno-fluorescent antibody assay (IFA) from the sera of 598 patients and nested polymerase chain reaction (PCR) tests using amplification of the 16S rRNA gene of A. phagocytophilum from 298 blood samples. In the serological IFA tests, 56 (9.4%) of 598 patients were positive for IgG or IgM of A. phagocytophilum (male: 28/354, 7.9% vs. female: 28/244, 11.5%). A high seropositive rate (45/56, 80.4%) was shown in patients ≥ 60 years. In the PCR tests, 25 of 298 patients showed a positive result.
This article reports laboratory results in relation to A. phagocytophilum from patients with suspected HGA in 2017.
Keywords: Anaplasmosis, Anaplasma phagocytophilum, Laboratory diagnosis
Lee Kyeong Ah, Hwang Seon Do, Kang Byung Hak, Kim Jae Ok
Division of Bacterial Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum. Clinical features of HGA range from mild illness such as fever, headache, myalgia, malaise, thrombocytopenia, and leukopenia to severe disease with gastrointestinal and respiratory distress, myocarditis, neurological complications, septic shock-like disease, and even death.
We performed serological HGA tests using immuno-fluorescent antibody assay (IFA) from the sera of 598 patients and nested polymerase chain reaction (PCR) tests using amplification of the 16S rRNA gene of A. phagocytophilum from 298 blood samples. In the serological IFA tests, 56 (9.4%) of 598 patients were positive for IgG or IgM of A. phagocytophilum (male: 28/354, 7.9% vs. female: 28/244, 11.5%). A high seropositive rate (45/56, 80.4%) was shown in patients ≥ 60 years. In the PCR tests, 25 of 298 patients showed a positive result.
This article reports laboratory results in relation to A. phagocytophilum from patients with suspected HGA in 2017.
Keywords: Anaplasmosis, Anaplasma phagocytophilum, Laboratory diagnosis
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