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Laboratory-based diagnosis of Japanese encephalitis in the Republic of Korea from 2013 to 2017
- Date2018-07-19 20:00
- Update2018-07-19 20:00
- DivisionDivision of Strategic Planning for Emerging Infectious Diseases
- Tel043-719-7271
Laboratory-based diagnosis of Japanese encephalitis in the Republic of Korea from 2013 to 2017
Lee EunJu, Lee Hyeokjin, Choi Wooyoung, Kang Chun
Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Lee Joo-Yeon Division of Emerging Infectious Disease and Vector Research, Center for Infectious Diseases Research, KNIH, KCDC
Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis. Since 2010, the number of patients with Japanese encephalitis (JE) in the Republic of Korea has increased. This article showed the results of the laboratory-based diagnosis of JE from 2013 to 2017. Real-time reverse tranion-polymerase chain reaction, enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and plaque reduction neutralization test were used to detect JEV in serum and/or cerebrospinal fluid (CSF) samples. Although most JE cases showed higher antibody titers in acute and convalescent serum samples, this study focused on investigating four unusual cases. Based on these results, if the initial sample from suspected JE patients was negative for JE IgM, the second and third samples from the same patient should be obtained to accurately diagnose of JEV infection.
Keywords: Japanese encephalitis, Japanese encephalitis virus, Enzyme-linked immunosorbent assay, Immunofluorescence assay, Plaque reduction neutralization test
Lee EunJu, Lee Hyeokjin, Choi Wooyoung, Kang Chun
Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Lee Joo-Yeon Division of Emerging Infectious Disease and Vector Research, Center for Infectious Diseases Research, KNIH, KCDC
Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis. Since 2010, the number of patients with Japanese encephalitis (JE) in the Republic of Korea has increased. This article showed the results of the laboratory-based diagnosis of JE from 2013 to 2017. Real-time reverse tranion-polymerase chain reaction, enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and plaque reduction neutralization test were used to detect JEV in serum and/or cerebrospinal fluid (CSF) samples. Although most JE cases showed higher antibody titers in acute and convalescent serum samples, this study focused on investigating four unusual cases. Based on these results, if the initial sample from suspected JE patients was negative for JE IgM, the second and third samples from the same patient should be obtained to accurately diagnose of JEV infection.
Keywords: Japanese encephalitis, Japanese encephalitis virus, Enzyme-linked immunosorbent assay, Immunofluorescence assay, Plaque reduction neutralization test
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