contents area
Public Health Weekly Report
detail content area
Introduction of reference materials for water- and food-borne disease viruses
- Date2018-02-28 22:34
- Update2018-02-28 22:34
- DivisionDivision of Strategic Planning for Emerging Infectious Diseases
- Tel043-719-7271
Introduction of reference materials for water- and food-borne disease viruses
Jung Sunyoung, Lee Deog-Yong, Choi Wooyoung, Kang Chun
Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Park Ye Eun
Division of Laboratory Diagnosis Management, Center for Laboratory Control of Infectious Diseases, KCDC
Positive controls are crucial to ascertain the reliability of results in diagnosis and experiments. Therefore, we have produced reference material according to international regulation and have used it as a positive control. In this article, we introduce the reference material for detection of water- and food-borne disease viruses. ISO guideline 34/35 is the international standard for reference material, which has the management-related, technical requirements for producing such materials. Reference material of norovirus, which is the representative water- and food-borne pathogen, has been produced globally. However, it remains valid only for a short duration after production and shows mismatches in amplification region. In Korea, some reference materials have been produced and are being used as internal controls without following the international guidelines. Therefore, the Korea Center for Disease Control and Prevention (KCDC) started to produce the reference material for water- and food-borne disease viruses based on the international standard, ISO guideline 34/35. The process of production was as follows: selection of the target region, RNA synthesis, measurement of concentration, contamination, homogeneity, and stability. First, we selected norovirus and subsequently have expanded to other food-borne disease viruses. Now, KCDC provides 6 type of reference materials to be used as positive control materials for diagnosis of viruses that cause water- and food-borne diseases. To improve the reference material produced, the standard materials obtained should be improved continuously and be used as positive controls for achieving the reliable experimental results.
Keywords: Reference Materials, Water and food-borne disease, Reliability, Diagnosis, Norovirus
Jung Sunyoung, Lee Deog-Yong, Choi Wooyoung, Kang Chun
Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, KCDC
Park Ye Eun
Division of Laboratory Diagnosis Management, Center for Laboratory Control of Infectious Diseases, KCDC
Positive controls are crucial to ascertain the reliability of results in diagnosis and experiments. Therefore, we have produced reference material according to international regulation and have used it as a positive control. In this article, we introduce the reference material for detection of water- and food-borne disease viruses. ISO guideline 34/35 is the international standard for reference material, which has the management-related, technical requirements for producing such materials. Reference material of norovirus, which is the representative water- and food-borne pathogen, has been produced globally. However, it remains valid only for a short duration after production and shows mismatches in amplification region. In Korea, some reference materials have been produced and are being used as internal controls without following the international guidelines. Therefore, the Korea Center for Disease Control and Prevention (KCDC) started to produce the reference material for water- and food-borne disease viruses based on the international standard, ISO guideline 34/35. The process of production was as follows: selection of the target region, RNA synthesis, measurement of concentration, contamination, homogeneity, and stability. First, we selected norovirus and subsequently have expanded to other food-borne disease viruses. Now, KCDC provides 6 type of reference materials to be used as positive control materials for diagnosis of viruses that cause water- and food-borne diseases. To improve the reference material produced, the standard materials obtained should be improved continuously and be used as positive controls for achieving the reliable experimental results.
Keywords: Reference Materials, Water and food-borne disease, Reliability, Diagnosis, Norovirus
This public work may be used under the terms of the public interest source + commercial use prohibition + nonrepudiation conditions