Analytical biochemistry, 2020.600, 113769-0, DOI: https://doi.org/10.1016/j.ab.2020.113769

Differential expressions of L1-chimeric trans in normal and matched-cancer tissues

Kim S, Shin W;Lee YM;Mun S;Han K

Abstract

L1s are a cis-regulatory elements and contain bidirectional internal promoters within the 5’ untranslated region (UTR). L1s provide bidirectional promoters that generate alternative trans and affect differential expressions in the human genome. In particular, L1 antisense promoters (L1ASPs) could produce aberrant trans in cancer tissues compared to normal tissues. In this study, we identified the L1-chimeric trans derived from L1ASPs and analyzed relative expression of L1-chimeric trans between normal and matched-cancer tissues.
First, we collected 425 L1-chimeric trans by referring to previous studies. Through the manual inspection, we identified 144 L1-chimeric trans derived from 44 L1 antisense promoters, suggesting that the antisense promoter acted as an alternative promoter. We analyzed relative gene expression levels of 16 L1-chimeric trans between matched cancer-normal tissue pair (lung, liver, gastric, kidney, thyroid, breast, ovary, uterus, and prostate) using real-time quantitative PCR (RT-qPCR) and investigated putative tranion factor binding motifs to determine activity of L1ASPs. Taken together, we propose that L1ASPs could contribute to the differential gene expression between normal and cancer tissues.

• DOI: https://doi.org/10.1016/j.ab.2020.113769
• ISBN or ISSN: 0003-2697

• 본 연구는 질병관리본부 연구개발과제연구비를 지원받아 수행되었습니다.
• This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.